569 papers
This study reveals that in large medaka embryos, the spatiotemporal reorganization of cytoplasmic dynein from a metaphase aster periphery halo to anaphase asters and organelles coordinately drives both aster growth and centrosome pulling to ensure proper cell division geometry.
This study identifies the Hippo signaling pathway as a conserved regulator of corneal endothelial regeneration, demonstrating that inhibiting this pathway to activate YAP promotes wound healing in both rodent and primate models, thereby offering a novel therapeutic target for treating corneal endothelial decompensation.
This study reveals that the Igf2-Igf binding protein complex, which is sequestered in neuroendocrine stem cells until injury releases it to drive proliferation via Rb repression, serves as a critical mechanism linking tissue repair to the initiation of small cell lung cancer.
This study reveals that cellular metabolostasis failure thresholds and toxicity mechanisms in *Saccharomyces cerevisiae* are determined by the interplay of metabolite network topology, chemical solubility, and a two-tiered translational control system that coordinates general resource conservation with metabolite-specific defense strategies to prevent amyloid-like aggregation.
This study proposes a novel three-stage AI-driven research paradigm that integrates adversarial training and non-equilibrium thermodynamics to evolve protein phase separation prediction from simple binary classification into a robust framework capable of physical mechanism analysis and the discovery of new membraneless organelles.
This study demonstrates that age-related stiffening of the ovarian stromal extracellular matrix impairs oocyte quality and granulosa cell-oocyte interactions by dysregulating TGF-β signaling, a dysfunction that can be rescued by activating this pathway.
The paper introduces SHOT-CCR, a biologically guided test-time adaptation framework that leverages cell-invariant gradient reversal to decouple morphological signals from batch effects, achieving state-of-the-art accuracy in classifying genetic perturbations across diverse Cell Painting datasets.
This study reveals that micron-scale endoplasmic reticulum (ER) discontinuities are a common, dynamic, and rapidly resolved feature of healthy *C. elegans* neurons that increase with age, stress, and specific genetic impairments, establishing a tractable model for investigating the molecular mechanisms of ER network maintenance in vivo.
This paper introduces ProtiCelli, a deep generative model trained on the Human Protein Atlas that synthesizes proteome-wide, spatially resolved images of 12,800 human proteins from just three landmark stains, thereby creating the "Proteome2Cell" dataset of 30.7 million virtual images to bridge the experimental scalability gap and enable comprehensive, single-cell spatial proteomics.
This study employs a genome-wide strategy to identify TXNDC15 as a novel, catalysis-independent essential factor in MARCHF6-mediated ERAD that regulates substrate exit from the ER and maintains lipid homeostasis.
This study establishes Chinese hamster ovary (CHO) cells as a robust model for investigating telomeric RNAs and reveals that ARIA transcripts, which originate from interstitial telomeric sequences, are essential regulators of telomeric DNA integrity by preventing the accumulation of single-stranded DNA at these sites.
This study introduces a non-invasive method using spontaneously engulfed elastic microbeads to reveal that 3D epithelial tissues generate localized, spatially segregated pulling and pushing forces through coordinated cytoskeletal dynamics, challenging the prevailing view of homogeneous force application.
This study establishes that airway epithelial homeostasis and stress adaptation in *Drosophila* are governed by a "Goldilocks principle" of JAK/STAT signaling, where balanced activity is essential for cell survival and integrity, while sustained overactivation drives pathological remodeling that is conserved across species and reversible via pharmacological inhibition.
This study demonstrates that integrating genetic code expansion with rational site selection allows for the minimally disruptive incorporation of the fluorescent noncanonical amino acid Anap into G3BP1 and TDP-43, enabling highly accurate visualization of their stress-induced dynamics and pathobiology in live cells and neurons without compromising protein function.
This study utilizes single-nucleus gene expression and eQTL analyses of the human retina, RPE, and choroid to demonstrate that specific AMD-risk variants alter gene expression in distinct cell types and reveal age-related declines in complement inhibitors within the choriocapillaris.
This study benchmarks three simple DNA staining-based image metrics for live-cell chromatin tracking, demonstrating that the newly introduced Diffuse Signal Index (DSI) offers superior sensitivity in distinguishing chromatin reorganization during NETosis compared to the coefficient of variation and 1-Gini, while all three correlate with chromatin accessibility.
This study reveals that the substrate diacylglycerol (DAG) promotes DGAT1 oligomerization and drives its localization to curved ER tubules, thereby modulating the enzyme's activity and subcellular distribution during triglyceride synthesis.
This study identifies small-molecule modulators that inhibit AHR, DOT1L, or GSK3 to dramatically enhance the efficiency and cytotoxic function of stroma-free, hiPSC-derived T cells, thereby establishing a robust strategy for scalable universal and autologous T cell therapies.
This study demonstrates that supplementing bull semen extenders with a low concentration (1%) of sustainably obtained blueberry extract significantly improves the post-thawing motility and quality of cryopreserved spermatozoa, offering a sustainable approach to enhancing reproductive outcomes in artificial insemination.
This study demonstrates that long-term mechanical confinement drives monocyte-to-macrophage differentiation across multiple cell types by activating the KDM6B enzyme to demethylate H3K27me3, thereby derepressing macrophage-specific transcriptional programs and enhancing phagocytic capacity.